10 sep. 2018 — Mona Österberg på Mercodia samt Martin och Christian Ryen på sig på Elisa-tester inom diabetes, fetma och kardiovaskulära sjukdomar.
Developed for both serum and plasma samples, the insulin and iso-insulin ELISAs are robust and highly reproducible. The human insulin and ultra-sensitive insulin products cover a sensitivity range from 0.15-200 mU/l, with no or insignificant cross-reactivity to human proinsulin or C-peptide, insulin analogues or rat/mouse insulin.
.. Statistical analysis All statistical analysis was performed using GraphPad Prism-6 software using either two-way ANOVA or Bonferroni test for p value calculations with > 95% confidence. Mercodia Feline Insulin ELISA is calibrated against an in house reference preparation of feline insulin. WARRANTY The performance data presented here was obtained using the procedure indicated. Any change or modification in the procedure not recommended by Mercodia AB may affect the results, in Address Mercodia AB, Sylveniusgatan 8A, SE-754 50 Uppsala, Sweden version 3.0 Phone +46 18 57 00 70 l Fax +46 18 57 00 80 l Vat No SE-556157510001 E-mail info@mercodia.com l Website www.mercodia.com Technical Note No: 34-0121 Substance Specificity Insulin ELISA Specificity Iso-Insulin ELISA Insulin, rat 0.7 % 71 % Insulin mouse 0.3 % 49 % termination of glucagon in rat, mouse, porcine and non- human primate (NHP) samples.
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Sample volume: 25 µL. Assay range: 0.025 - 1.0 μg/L. Detection limit: ≤ 0.025 µg/L. Incubation: 120 + 30 min. A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media. The Mercodia insulin assays have little or no cross-reactivity to insulin analogs or to rat or mouse insulin and comes with full cross-reactivity data. The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.
It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Mercodia Ultrasensitive Mouse Insulin ELISA provides a method for the quantitative determina-tion of insulin in mouse serum or plasma.
The Mercodia insulin assays have little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse models. Advantages - Calibrated against 1st International Reference Preparation 66/304 for human insulin
Sweden). Insulin tolerance tests were similarly performed in mice fasted for 5 h using a single 2014. 55: 516–523. Supplementary key words cholesterol • glucose homeostasis • insulin the Mouse Insulin ELISA (Mercodia, Uppsala, Sweden).
and in the rates of conversion to insulin (3). Increased proinsulin over insulin ratio may be found in rat and mouse models of hyperglycemia (4,5), or after manipulation of proinsulin-processing enzymes (6,7). The Mercodia Rat/Mouse Proinsulin ELISA is specific for proinsulin and does not cross-react with insulin or c-peptide in rat or mouse.
13) To get total insulin, add 100 uL of lysis buffer. 14) For ELISAs, we use Mercodia Mouse Insulin ELISA kits -- typically I highly recommend Mercodia (Sweden) insulin ELISA Kit since it detects very recognize insulin or glucagon from MIN6 cells because their origin is mouse. 4131 products Compare Insulin ELISA Kits from leading suppliers on Biocompare. Mouse Insulin (INS) ELISA Kit. Detection Target: Insulin. Detection Range:. 1 Sep 2016 mined with mouse insulin ELISA kit (Mercodia) and normalized to the total protein content. 2.7.
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Manufacturer: Mercodia Inc 10111301 A high quality enzyme immunoassay for specific quantification of human insulin in serum, plasma, or cell culture media. The Mercodia insulin assays have little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse
Mercodia Feline Insulin ELISA is calibrated against an in house reference preparation of feline insulin. WARRANTY The performance data presented here was obtained using the procedure indicated. Any change or modification in the procedure not recommended by Mercodia AB may affect the results, in
Insulin, Mouse, ELISA Kit, Ultrasensitive, Mercodia (96well) 文献数:3. SDS; メーカーサイト; 使用文献; お問い合わせ; 商品コード:10-1249-01; メーカー:MRD; 包装:1kit; 価格:¥66,000 在庫:3個以上
This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research. - Find MSDS or SDS, a COA, data sheets and more information.
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- High sensitivity - Assay range 0.025 - 1.5ug/L. - Fast and easy-to-use. Safety information: The Merocodia Insulin ELISA is a two-site enzyme immunoassay utilizing the direct sandwich technique with two monoclonal antibodies directed against separate antigenic determinants of the insulin molecule. Specimen, control, or standard is pipetted into the sample well, then followed by the addition of peroxidase-conjugated anti-insulin antibodies.
- Find MSDS or SDS, a COA, data
Insulin ELISA Kit provides a method for the quantitative determination of human insulin in serum or plasma. As with all diagnostic tests, a definitve clinical
Mercodia Mouse Insulin ELISA. Datum: 2017-07-06.
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SUMMARY AND EXPLANATION OF THE TEST Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthe-sized in the ß-cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin.